Product center

Yocon is committed to produce high-quality and brand serum-free cell culture products, laying a solid foundation for cell culture media solutions.

Mature NK Cell Cryopreservation Solution

· Recombinant human albumin system · High post-thaw recovery & high cell viability · Maximal retention of NK cytotoxicity

Product use

Compatible with 30 mL cryobag large-volume cryopreservation. At high densities of 2E7 and 5E7 cells/mL, cord blood NK cells achieve >80% recovery with stable phenotype. Peripheral blood NK cells also show excellent post-thaw performance, with well-preserved cytotoxicity and NK phenotypes, suitable for closed manufacturing using cryobags.
Product video


Product NameCatalog No.SpecificationStorage ConditionShelf Life
Mature NK Cell Cryopreservation SolutionNC0604-1500 mL/ bottle2–8 ℃, protected from light12 months
NC0604-2100 mL/ bottle

Product Advantages

  • This formulation adopts a recombinant human albumin system, serum-free, human-origin & animal-origin free, with fully chemically defined components. The low-DMSO formula significantly reduces cytotoxicity, suitable for long-term preservation of NK cells induced from peripheral blood, umbilical cord blood and other sources.


  • Special membrane and protein stabilizers are incorporated to effectively protect cells from cryoinjury. Post-thaw recovery can reach over 80%, and the viability difference from fresh control is minimal (<2%). Meanwhile, the high cytotoxic activity of NK cells is maintained.


  • Ready-to-use, no extra preparation required, easy to operate; supports direct cryopreservation at large volume and high cell density.


Umbilical Cord Blood-derived NK Cryopreservation Data


Cryovial — Short-term Cryopreservation


image.png


Cryovial — 3 Months Cryopreservation





image.png



For NK cells derived from umbilical cord blood samples cryopreserved in cryovials, the recovery rate remains >80% for both short-term and long-term cryopreservation. The viability difference compared with fresh samples is <1%; cytotoxicity (E:T=2:1) retains more than 80% of fresh samples. The CD3⁻CD56⁺ phenotype is basically consistent with fresh samples, and CD56⁺CD16⁺ phenotype retains over 80% of fresh samples. The product outperforms competing cryopreservation media.


30 mL Cryobag Cryopreservation


image.png



When NK cells from umbilical cord blood samples are cryopreserved in 30 mL cryobags, the recovery rate >80%, viability difference <1% relative to fresh samples, and the CD3⁻CD56⁺ phenotype shows no significant difference from fresh samples.


Peripheral Blood-derived NK Cryopreservation Data


image.png


For NK cells derived from peripheral blood samples cryopreserved in cryovials, the short-term recovery rate exceeds 80%, viability difference <2% compared with fresh samples; cytotoxicity (E:T=2:1) retains more than 70% of fresh samples. The CD3⁻CD56⁺ phenotype is basically consistent with fresh samples, and CD56⁺CD16⁺ phenotype retains over 70% of fresh samples. The product outperforms competing cryopreservation media.



Product advantages

Data download