| Product Name | Cat. No. | Specification | Storage Temperature |
|---|---|---|---|
| Immune Cell Serum-Free Medium 6.0 (rHSA) | NC0114 | 1000 mL/bottle | 2～8 ℃ |
Product Advantages
Fully serum-free, human-origin-free and animal-origin-free with chemically defined composition, reducing regulatory submission difficulty and quality control cost.
Functionalized recombinant human albumin system, which significantly restores and enhances the biological potency of NK cells.
Supports high-density expansion up to 5E6/mL, suitable for process development of NK therapeutics and CAR-NK.
Performance Highlights
When combined with Yocon NK Induction & Expansion Kit, the expansion fold can reach 1000~2000 times;
The positive rate of CD3⁻CD56⁺ remains stably >90%
Compatible with peripheral blood and umbilical cord blood samples, works for both cryopreserved and fresh samples
Data Summary
Peripheral Blood Samples
| Sample NO. | Cryopreserved | Seeding Density | Final Volume | Culture Duration | Expansion Fold | Harvested Cells | NK Phenotype |
|---|---|---|---|---|---|---|---|
| Sample 1 | Cryopreserved | 2×10⁷ | 2000 mL | 17 days | 504 | 1.0×10¹⁰ | 95.42% |
| Sample 2 | Fresh | 2×10⁷ | 2500 mL | 16 days | 646 | 1.29×10¹⁰ | 98.69% |
Conclusion: Peripheral blood samples (including cryopreserved samples) cultured with this product can achieve over 500-fold expansion, with NK purity stably above 95%.
Umbilical Cord Blood Samples
| Sample NO. | Transportation Time | Cryopreserved | Anticoagulant Ratio | Seeding Cell Number | Final Volume | Culture Duration | Expansion Fold | Harvested Cells | NK Phenotype |
|---|---|---|---|---|---|---|---|---|---|
| Sample 1 | ~24 h | Fresh | 18.6% | 8×10⁶ | 1920 mL | 23 days | 709 | 5.68×10⁹ | 98.45% |
| Sample 2 | ~24 h | Fresh | 14.7% | 2×10⁷ | 3735 mL | 25 days | 1127 | 2.25×10⁹ | 97.05% |
Conclusion: For umbilical cord blood samples after 24 h transportation cultured with this medium, the expansion fold can reach 700~1100 times, and NK purity remains stably above 97%.
Comparison Data: This Product vs NK5.0 (BSA)
| Comparison Item | NK5.0 (BSA) | Immune 6.0 (rHSA) | Improvement |
|---|---|---|---|
| Cell Density /mL | 4E6–5E6 | 5E6–6E6 | ~20% increase |
| Expansion Fold | Dependent on initial seeding density & final culture volume | ~10%–30% increase | |
| NK Phenotype Stability | 95%–99% | 95%–99% | Comparable |
Conclusion: Under identical culture conditions, Immune 6.0 (rHSA) achieves or outperforms NK5.0 (BSA) in expansion fold and cell yield, while upgrading safety profile with complete animal-origin-free formulation.
Comparison of Expansion Fold and Positive Rate with Competitors
Conclusion: Under identical culture conditions (all paired with Repair 1.0), Yocon Immune 6.0 (rHSA) exhibits prominent advantages in expansion fold compared with competitive brands, while NK purity is maintained at a comparable level, achieving the performance breakthrough of Higher Yield with Equivalent Purity.
