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High-Purity NK Series Serum-free Culture Kit

YoukangBio NK Cell Serum-Free Expansion Kit is developed for activation and large-scale expansion of NK cells derived from umbilical cord blood and peripheral blood. It delivers excellent process fault tolerance, supporting culture of unconventional samples, long-transport samples and culture protocols without autologous plasma, and efficiently recovers and activates damaged cells. It achieves >200-fold expansion within 14–16 days with high NK phenotype purity. Expanded NK cells exhibit potent and stable cytotoxicity against K562 target cells. The culture success rate exceeds 90% with favorable batch-to-batch consistency, compatible with CMC requirements for IIT and IND programs, serving as a reliable culture platform for allogeneic NK drug development.

Product use

The High-Purity NK Series Culture Kit is optimized and upgraded for more challenging sample conditions. For low-quality samples (initial positive rate <10%, transportation over 8 hours, unknown cryopreservation quality, no autologous plasma, etc.), it significantly improves culture success rate. Suitable for pre-clinical research or translational medicine teams with high requirements for positive rate and stability.
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Product Specifications


Kit ClassificationProduct NameCat.No.SpecificationBrief Description
High-Purity NK Kit (50mL/2L)

Immune Cell Repair Culture MediumNC0102.F200 mL / bottleFor most samples, the positive rate can be stably maintained at 80–95%, with a total cell yield of 8–10 billion. Suitable for peripheral blood, umbilical cord blood and cryopreserved PBMC samples. Requires 50 mL sample, inoculate into one T75 flask with 30 mL medium. Used together with Recovery Medium 1.0.
NK Cell Serum-free Medium 5.0NC01021000 mL / bottle
Standard NK Reagent Kit (50 mL)AN0107.2For 50 mL sample
High-Purity NK Kit (30mL/2L)

Immune Cell Repair Culture MediumNC0102.F200 mL / bottleFor most samples, the positive rate can be stably maintained at 80–95%, with a total cell yield of 8–10 billion. Suitable for peripheral blood, umbilical cord blood and cryopreserved PBMC samples. Requires 30 mL sample, inoculate into two T75 flasks with 20 mL medium. Used together with Recovery Medium 1.0.
NK Cell Serum-free Medium 5.0NC01021000 mL / bottle
Standard NK Reagent Kit (30 mL)AN0107For 30 mL sample


Product Advantages


  • High fault tolerance: Supports NK cell activation and culture from unconventional umbilical cord blood or peripheral blood samples; compatible with culture protocols without autologous plasma.


  •  High performance: Achieves >200-fold cell expansion within 14–16 days, with ≥85% positive rate. 


  • High stability: Markedly improves recovery and activation efficiency of damaged cells; culture success rate exceeds 90%, less susceptible to initial sample quality.



Performance Data


1. Culture Cases


Umbilical Cord Blood Samples


Sample NO.Transportation TimeCryopreservedAnticoagulant RatioSeeding Cell NumberFinal VolumeCulture DurationExpansion FoldHarvested CellsNK Phenotype
Sample 1~24 hCryopreserved21.5%6×10⁷4 L26 days25315.2×10⁹99.05%
Sample 2~24 hFresh18.7%6×10⁷6.3 L27 days42825.7×10⁹98.54%
Sample 3~24 hCryopreserved25.0%2×10⁷6.3 L28 days3707.4×10⁹98.67%
Sample 4~24 hFresh20.0%1.2×10⁸12.9 L29 days40848.9×10⁹99.22%
Sample 5~24 hFresh19.0%6×10⁷6.12 L28 days35121.0×10⁹97.52%


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Umbilical Cord Blood Samples



Sample NO.CryopreservedSeeding VolumeFinal VolumeCulture DurationHarvested CellsNK Phenotype
Sample 1Fresh30 mL2 L16 days6.95×10⁹97.71%
Sample 2Fresh30 mL2 L16 days8.14×10⁹98.87%
Sample 3Fresh20 mL2 L14 days6.37×10⁹93.71%
Sample 4Fresh20 mL2 L14 days7.76×10⁹95.23%


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2. Cytotoxicity of NK Cells against K562


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Product advantages

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