Product Specifications

Kit Classification	Product Name	Cat.No.	Specification	Brief Description
Standard NK Kit (50 mL / 2 L)	Immune Cell Repair Culture Medium	NC0102.F	200 mL / bottle	For most NK samples with high initial phenotype, the positive rate can be stably maintained between 50–80%, cell yield reaches 8–10 billion. Applicable to peripheral blood, umbilical cord blood and frozen PBMC samples. Requires 50 mL sample, seeded in T75 flasks with 30 mL culture medium. Compatible with Repair 1.0 Medium.
	NK Cell Serum-free Medium 5.0	NC0102	1000 mL / bottle
	Standard NK Reagent Kit (50 mL)	AN0107.2	For 50 mL sample
Standard NK Kit (30 mL / 2 L)	Immune Cell Repair Culture Medium	NC0102.F	200 mL / bottle	For most NK samples with high initial phenotype, the positive rate can be stably maintained between 50–80%, cell yield reaches 5–8 billion. Applicable to peripheral blood, umbilical cord blood and frozen PBMC samples. Requires 30 mL sample, seeded in two T75 flasks with 20 mL culture medium. Compatible with Repair 1.0 Medium.
	NK Cell Serum-free Medium 5.0	NC0102	1000 mL / bottle
	Standard NK Reagent Kit (30 mL)	AN0107	For 30 mL sample

Product Advantages

Induced by purified factors, no feeder cells required. Simple and reproducible culture workflow with consistent cell status. Medium replenishment following recommended protocol, no routine cell counting needed.
For normal blood samples, ≥180-fold expansion and ≥50% positive rate can be achieved within 14–16 days of culture using the standard protocol.
The basal medium (NC0102) has completed US FDA DMF filing, DMF No.: 37874.

Sample No.	Sample Information	Seeding Cell Count	Final Culture Volume	Culture Duration	Harvested Cell Count	NK Phenotype
Sample 1	Cryopreserved PBMC	4E7	2.2 L	14 days	9.97×10⁹	69.02%
Sample 2	Fresh PBMC	4E7	2.2 L	14 days	8.45×10⁹	71.37%
Sample 3	Cryopreserved PBMC	4E7	2.2 L	14 days	10.85×10⁹	97.11%
Sample 4	Fresh PBMC	4E7	2.2 L	14 days	7.02×10⁹	86.16%