Product Name | Packaging | Storage | Shelf Life | Catalog No. |
| iPSC Serum-Free Medium Base | 400mL/bottle | 2-8 ℃, protected from light | 12 个月 | NC0401 |
| iPSC Serum-Free Medium Supplement | 100mL/bottle | -20℃, protected from light | 12 个月 | NC0401.S |
Product Advantages
Serum-free medium with fully defined chemical composition. Optimized formulation uses thermostable cytokines for enhanced buffering capacity.
Supports medium change every other day and single-cell passaging; simple operation and high applicability.
Superior expansion performance; enables >50 passages of feeder-free culture with normal karyotype and no obvious differentiation.
Performance Data
1. Cell Proliferation
Robust expansion: In 6-well plates, average expansion fold reaches ~20 within a 4-day culture cycle, and exceeds 30 over 5 days.
Long-term stability: Over 50 passages of feeder-free culture, cell harvest yield remains high.
2. Pluripotency Maintenance
Stable marker expression: High levels of pluripotency markers are retained at high passages（TRA-1-60 ≥90%, Oct4 ≥95%）.
| Group | TRA-1-60 | Oct-4 |
|---|---|---|
| P5 | 98.72% | 97.63% |
| P15 | 94.48% | 99.59% |
| P30 | 93.47% | 99.73% |
| P50 | 99.10% | 99.53% |
Triploblastic differentiation potential:iPSCs cultured up to Passage 25 can still successfully differentiate into three germ layers, with significant upregulation of marker mRNA for each germ layer
3.Cell Morphology
At P50, cells retain typical iPSC morphology (round/oval, clear boundaries, cobblestone-like colonies). No obvious differentiation or senescence during long-term culture; colony architecture remains intact.
4.Cell Morphology
Normal karyotype: G-banded karyotyping shows no abnormalities at P5, P10, P40 and under different medium-change schedules.
Genetic stability: Genome integrity maintained in long-term culture, meeting criteria for high-quality iPSC culture.
5.Differentiation & Application Potential
Differentiable into iMSCs: P50 iPSCs successfully differentiate into mesodermal cells and further yield iMSCs with internationally consistent phenotypes.
Multiple culture workflows supported: 4 medium-exchange protocols available; consistent cell phenotype, morphology and expansion across workflows
